@article{Wenz:2020:10.12688/f1000research.22464.1,
author = {Wenz, R and Conibear, E and Bugeon, L and Dallman, M},
doi = {10.12688/f1000research.22464.1},
journal = {F1000Research},
title = {Fast, easy and early (larval) identification of transparent mutant zebrafish using standard fluorescence microscopy [version 1; peer review: 2 approved]},
url = {http://dx.doi.org/10.12688/f1000research.22464.1},
volume = {9},
year = {2020}
}

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TY  - JOUR
AB  - The availability of transparent zebrafish mutants (either TraNac: tra b6/b6; nac w2/w2 or casper: roy a9/a9; nac w2/w2 ) for live imaging studies together with the ease of generating transgenic lines are two of the strengths of the zebrafish model organism. The fact that transparent casper ( roy a9/a9;nac w2/w2) and silver nacre ( nac w2/w2) mutants are indistinguishable by eye at early stages (1-5 days post-fertilization; dpf) means many fish must be raised and later culled if they are not transparent. To identify translucent mutants early and easily at the early larval stage (≤5 dpf) before they are classified as protected animals, we developed a simple screening method using standard fluorescence microscopy. We estimate that this procedure could annually save 60,000 animals worldwide.
AU  - Wenz,R
AU  - Conibear,E
AU  - Bugeon,L
AU  - Dallman,M
DO  - 10.12688/f1000research.22464.1
PY  - 2020///
SN  - 2046-1402
TI  - Fast, easy and early (larval) identification of transparent mutant zebrafish using standard fluorescence microscopy [version 1; peer review: 2 approved]
T2  - F1000Research
UR  - http://dx.doi.org/10.12688/f1000research.22464.1
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32934809
UR  - http://hdl.handle.net/10044/1/83118
VL  - 9
ER  - 

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